[ Beanref ]

Cytogenetics in Phaseolus and Vigna

- contributed for BeanRef by Dr. M. Nenno -

Chromosomes

Mitotic chromosomes

Genus
Phaseolus
Vigna
Chromosome number
2n = 22 (Karpechenko, 1925)
Chromosome size 0.85 µm (P. ricciardianus) to
3 µm (P. vulgaris) (Sarbhoy 1978)		 1.6 µm and 3.7 µm
(Sen and Bhowal, 1960
Karyotype
  • Moscone et al. (1999)
  • Zheng et al. (1991)
  • Sinha and Roy (1979)
  • Galasso et al. (1992; 1993)
  • Zheng et al. (1991)

Polytene chromosomes

See also ...

Nuclear DNA amount/content (2C)

The following data are taken from Bennett and Leitch (1995)[1] and Nagl and Treviranus (1995)[2]:
DNA content Phaseolus Vigna
Minimum 0.9 pg P.macvaughi [1] 1.0 pg V.oblongifolia [1]
Maximum 2.18 pg P.pluriforus [2] 1.3 pg V.unguiculata [1]
Mean 1.55 pg wild genotypes [2] 
1.49 pg cultivars [2]		 1.13 pg [1]

Further cytogentic studies

Molecular cytogenetics/ In situ hybridization

Table: Reports of in situ hybridizations on polytene and metaphase chromosomes of Phaseolus and Vigna. (R) = Probe labeled radioactively (3H or 35S)
Phaseolus Vigna
Probe Polytene Chr.
Mitotic Chr.
Polytene Chr.
Mitotic Chr.
High and middle repetitive sequences
Fast renaturating
sequences and
satellite seq. 		Durante et al. (1987)(R)
-
-
-
Centromeric
sequence
-
-
-
 Galasso et al. (1995)
Ty1-copia-like 
retrotransposable element
-
-
-
 Galasso et al. (1997)
rDNA
(5S and/or
18S-25S)		 Avanzi et al. (1972)(R)
Brady and Clutter (1972)(R)
Schumann et al. (1994)(R)
Nenno et al. (1994)
Guerra et al. (1996)
Nenno M. (1998)		 Zheng et al. (1994)
Shi et al. (1996)
Moscone et al. (1999)
Pedrosa et al. (2003)
Guerra et al. (1996)		 Zheng et al. (1994)
Galasso et al. (1995)
Guerra et al. (1996)
Minisatellites Nenno et al. (1996)
Nenno M. (1998)
-
-
-
Microsatellites
or SSRs		 Nenno et al. (1998a)
Nenno M. (1998)
-
  
-
-
Telomere Nagl (1991)
Guerra and Kenton (1996)
Nenno et al. (1998b)		 Guerra and Kenton (1996)
-
 Galasso et al. (1995)
Low-copy sequences
Phaseolin Schumann et al. (1990)(R)
Nenno et al. (1994)
-
-
-
PGIP gene Frediani et al. (1993)(R)
-
-
-
Leghemoglobin 
(Lb) gene		  
-
 Uchiumi et al. (1998)  
-
-
RFLP Clones   Pedrosa et al. (2003    

References