Cytogenetic map
In order to describe chromosomal localization of FISH signals chromsomes have been further divided artificially in areas, even without necessarily showing physical differences in staining intensity, color, or structure. This additional division is indicated using a single apostroph after the area number (e.g. 1', 2' or 3'). So 1' stands for the proximal, 2' the central and 3' the distal chromsome segment. Example: Aq11.2' means the central segment of the section Aq11.
The table below contains to loci of the hybridiziations with different probes. Under this is reported the first cytogentic map of the polytene chromosomes of the embryo suspensor of Phaseolus coccineus. From the total number of 57 loci of microsatellite movties (SSR), 25 (10 motives) are located in euchromatin while 32 (10 motives) were found in heterochromatin. Signals inside the NOR have not taken into consideration. Loci in heterochromatin regions are underlined. DNC = dense nucleolar chromatin. * = region of condensed chromatin of the NOR
DNA probes | loci of FISH signals |
---|---|
18S-25S | Ap2,Iq3, Kp2 |
5S | Iq12,Kq25 |
Phaseolin | Gp21.3' |
(AT)8 | Aq21.2', Aq31, Bq21, Bqter, Hq21 |
(AG)8 | Aq21.3',Aq31, Bp21.2', Bq21, Bq31.2',
Cp12, Cq21, Cq31.2',Kq22-23 |
(CA)8 | Ap2*, Bq21, Bpter, Iq3*, Kp2* |
(AAT)5 | Aq21.2',Aq21.3', Bq21 |
(AAG)5 | Aq32,Bp21.1', Bq21, Bq31.1', Ip22, Kq22 |
(CAC)5 | Ap2*,Iq3*, Kp2* |
(AATG)4 | cHC all chromsomes and DNC |
(GATA)4 | Aq21.1',Aq21.3', Bq21 |
(GACA)4 | Aq21.3',Bq21 |
(C)16 | Aq32,Bp21.1', Bq31.1', Ip31, Jp22-24,
Jq22-23, Hp11-12,Hq11, Hq22-24 |
(GC)8 | Kq31.3' |
(GCC)5 | Aq31, Bp21.2', Bq21, Cp12, Cq21-22, Kq22 |
Telomer-Sequenz | p/qter all chromsomes |
M13 consensus sequence | cHC all chromsomes and DNC |
33bp repeat consensus sequence | p/qter all chromsomes |